Translational_Unit
cmpA

Part:BBa_K2078000:Design

Designed by: Michael O   Group: iGEM16_CLSB-UK   (2016-10-06)


cmpA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 95
    Illegal AgeI site found at 128
    Illegal AgeI site found at 281
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-Part was synthesized to already contain RBS. -Scar between RBS and ORF is TACTAG. -The part was synthesized with with BioBrick prefix and suffix. -EcoR1 site was preceded by CCG to allow us to clone the part into pSB1C3. -


Source

De-novo synthesis by IDT.

References